LOCUS       40924_22814        5667 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGV1025 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Broeders S, De Greve H, De Rycke R, Bauw G, Jansens S, Buys L, 
            Smagghe G, Ripoll C, Peumans WJ, Van Damme EJM., Hernalsteens JP.
  TITLE     Leaf and bulb lectins from garlic (Allium sativum L.) are expressed,
            correctly processed and targeted to the cytoplasm in transgenic 
            tobacco plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   De Greve H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2001) De Greve H., Genetische Virologie, Vrije 
            Universiteit Brussel, Paardenstraat 65, Sint-Genesius-Rode, B-1640, 
            BELGIUM
REFERENCE   3  (bases 1 to 5667)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5667)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2001) De Greve H., Genetische Virologie, Vrije Universiteit 
            Brussel, Paardenstraat 65, Sint-Genesius-Rode, B-1640, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        910..1255
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1294..3102
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      3176..3428
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3446..3462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3470..3486)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3494..3524)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3539..3560)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3848..4436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4610..5467)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5468..5572)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcc
      421 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
      481 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
      541 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
      601 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
      661 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
      721 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
      781 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
      841 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
      901 aagggcaatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      961 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     1021 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     1081 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     1141 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1201 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     1261 gactctagag gatccccggg tggtcagtcc cttatgttac gtcctgtaga aaccccaacc
     1321 cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga
     1381 attgatcagc gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc
     1441 agttttaacg atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat
     1501 cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat
     1561 gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc
     1621 ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt
     1681 atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt
     1741 accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga
     1801 atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg
     1861 gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat
     1921 ggtgatgtca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc
     1981 actagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc
     2041 tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc
     2101 ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac
     2161 tttactggct ttggtcgtca tgaagatgcg gacttacgtg gcaaaggatt cgataacgtg
     2221 ctgatggtgc acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg
     2281 cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt
     2341 gatgaaactg ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac
     2401 aagccgaaag aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta
     2461 caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt
     2521 attgccaacg aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg
     2581 gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc
     2641 gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac
     2701 ggatggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt
     2761 ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg
     2821 ttagccgggc tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg
     2881 ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg
     2941 aatttcgccg attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg
     3001 atcttcactc gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact
     3061 ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac aatgaatcaa caactctcct
     3121 ggcgcaccat cgtcggctac agcctcggga attgctaccg agctcgaatt tccccgatcg
     3181 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     3241 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     3301 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     3361 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     3421 actagatcgg gaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4021 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4261 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5221 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5581 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     5641 ataggcgtat cacgaggccc tttcgtc
//
